Berta Canal Domenech and Claudia Fricke - Thermal sensitivity of fitness traits and reproductive tissues across four Drosophila species published in Journal of Thermal Biology 135 (2026) 104352; https://doi.org/10.1016/j.jtherbio.2025.104352 The attached four files, as listed below, contain the raw data as collected from the experiments described in Canal Domenech & Fricke 2026. 1) Offspring number 2) Recover time 3) Accessory gland size 4) Sperm presence Detailed description of the data contained in the individual files. 1) Offspring number Male_ID individual male identifier Temperature temperature treatment males experienced during development given as °C Day test day (= male age) post-eclosion Offspring_num number of offspring produced within 24 hours Species Drosophila species measured: D. melanogaster, D. mauritania, D. simulans and D. obscura 2) Recovery time Male_ID individual male identifier Develop_temp temperature treatment males experienced during development given as °C Species Drosophila species tested: D. melanogaster, D. mauritania, D. simulans and D. obscura Recovery_t recovery time in hours New_temp temperature coded as increases of 1°C temperature steps from the control temperature Status score whether a male recovered (1-) or not (0-) 3) Accessory gland size Male_ID individual male identitfier Temperature temperature treatment males experienced during development given as °C Day test day (= male age) post eclosion Area1 area of the first accessory gland in µm Area2 area of the second accessory gland in µm Wing_L1 length of the first wing in µm Wing_L2 length of the second wing in µm Species Drosophila species measured: D. melanogaster, D. mauritania, D. simulans and D. obscura 4) Sperm presence Male_ID individual male identifier Temperature temperature treatment males experienced during development given as °C Day test day (= male age) post eclosion T1 sperm presence (1- present, 0- absent) in the first testis T2 sperm presence (1- present, 0- absent) in the second testis Species Drosophila species scored: D. melanogaster, D. mauritania, D. simulans and D. obscura